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KMID : 1144320100420050291
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감염과 화학요법
2010 Volume.42 No. 5 p.291 ~ p.295
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Effects of Peroxisome Proliferator-Activated Receptor-γ on the Production of Tumor Necrosis Factor-α in Stimulated Human Monocoytes
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Kwon Eun-Young
Park Chul-Min
Kwon Jae-Cheol
Kim Si-Hyun
Park Sun-Hee
Choi Su-Mi
Lee Dong-Gun
Yoo Jin-Hong
Choi Jung-Hyun
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Abstract
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Background:We evaluated the effects of peroxisome proliferator-activated receptor-γ (PPAR-γ) on the production of tumor necrosis factor-α (TNF-α) and expression of nuclear factor-κB (NF-κB) in stimulated THP-1 cells, a human monocyte cell line.
Materials and Methods :We evaluated the cytotoxic effect of 15-Deoxy-Δ12,14- prostaglandin J2 (15d-PGJ2), one of natural PPAR-γ ligands, using commercial cell proliferation assay. Cells were pretreated with 15d-PGJ2 and then stimulated with lipopolysaccharide (LPS) or lipoteichoic acid (LTA). The amount of TNF-α was measured by using commercial ELISA method. NF-κB activation was evaluated by Western blot analysis.
Results:15d-PGJ2 showed dose-dependent cytotoxic effect on the tested cells after 4 hr of treatment. Stimulation of cells by LPS or LTA induced TNF-α production. TNF-α production was markedly decreased in the cells pretreated with 15d-PGJ2compared to cells treated only with LPS or LTA in a dose-dependent manner. Pretreatment of 15d-PGJ2 reduced LPS or LTA induced NF-κB expression in the nuclear extracts of THP-1 cells.
Conclusion: 15d-PGJ2 pretreatment decreased TNF-α production from the THP-1 cells stimulated by LPS or LTA, and this assumed to be associated with inhibition of NF-κB activation.
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KEYWORD
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Peroxisome proliferator-activated receptor-γ (PPAR-γ), Tumor necrosis factor (TNF)-α, Nuclear factor-κB (NF-κB)
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